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Pointwise SE for NLME

To: <s-news@lists.biostat.wustl.edu>
Subject: Pointwise SE for NLME
From: Rasmus Ejrnæs <as-science@greennet.gl>
Date: Fri, 2 Dec 2005 09:29:06 -0300
Importance: Normal
Organization: Arktisk Station
Reply-to: <as-science@greennet.gl>

Dear S-plussers

 

I have fitted a non-linear regression in the form of a logistic growth function to experimental data. I found significant effects of my treatment (a gradient in temperature increase at soil surface) on the f1 parameter describing the asymptote (Asym.). This fixed-effect was best modelled as a quadratic term within each of the three species subjected to experimentation:

 

Fixed effects: list(Asym ~ Species:fati^2, xmid ~ Species:fati + micro, scal ~ 1)

                                 Value    Std.Error  DF      t-value p-value

         Asym.(Intercept)   8.60263485 0.5743440284 567  14.97819151  <.0001

Asym.SpeciesCeraI(fati^2)  -0.02873649 0.0218307696 567  -1.31632989  0.1886

Asym.SpeciesPolyI(fati^2)  -0.07381521 0.0218344296 567  -3.38067979  0.0008

Asym.SpeciesSaxiI(fati^2)  -0.04759965 0.0218275779 567  -2.18071160  0.0296

 

The reported standard errors are for the coefficients of the model, but my follow-up research question regards what temperature increase results in a significant response of the species, and for this I reckon that I need the pointwise confidence intervals. I have not been able to find coverage of the issue of pointwise error estimation in the lme/nlme documentation and also not in the Pinheiro & Bates book.

 

I am very little of a statistical expert - I can manage to calculate the pointwise CI if I assume that I can treat the fixed-effects model as a simple linear regression and forget that it is embedded in a non-linear model. But my confidence in doing so is not sufficient for a scientific publication! I also guess that it is less simple – else, it would have been natural to implement the se.fit=T option in the predict.nlme-function just like predict.lm. (?)

 

I would appreciate your advice on this.

 

Regards

 

Rasmus

 

Rasmus Ejrnæs

Associate Professor

Arctic Station

3953 Qeqertarsuaq

www.nat.ku.dk/as

 

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